Biosynthesis of Acridone Alkaloids. A Cell-Free System from Ruta graveolens Cell Suspension Cultures
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چکیده
Ruta graveolens, Suspension Cultures, Cell-Free System, Acridone Biosynthesis, 1,3-DihydroxyN-methylacridone A cell-free extract of Ruta graveolens cell suspension cultures, strain R-20, was prepared which synthesizes 1,3-dihydroxy-N-methylacridone from N-methylanthranilic acid and malonylcoenzyme A. The reaction is strongly dependent on the presence of ATP. After adding of coenzyme A to the assay mixtures a second compound X2 is accumulated to a considerable extent. The structure of this substance has not yet been clarified. Apparently X2 is a precursor of 1,3-dihydroxy-N-methylacridone, the key intermediate in the pathway leading to more complex acridones.
منابع مشابه
Purification and properties of acridone synthase from cell suspension cultures of Ruta graveolens L.
Acridone synthase has been purified from cell suspension cultures of Ruta graveolens using a combination of gel filtration and ion exchange chromatography. The purified enzyme has an apparent molecular weight of 69 kDa on gel filtration and a subunit structure on SDS-PAGE of 40 kDa. The apparent Km-values are 10.64 microM and 32.8 microM for N-methylanthraniloyl-CoA and malonyl-CoA, respectivel...
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